1. Centrifuge 20 mL of DLF_R004 E. coli culture, resuspend cells in lysis buffer. 2. Freeze cells for 1 hour at -80°C, thaw at 37°C for 1 hour. 3. If not using DLF_R004, lyse cells with lysis buffer, lysozyme, and freeze-thaw cycles. 4. Prepare serological pipette by cutting at the 3 mL mark, sealing bottom with parafilm, and testing for leaks with 1 mL of water. 5. Secure serological pipette to a vertical surface. 6. Fill pipette with at least 2.5 mL cell lysate, measure distance from 2 mL to 1 mL mark. 7. Position cell phone camera to record pipette, drop a glass bead inside, repeat two more times. 8. Stop recording, remove parafilm seal, empty and rinse pipette, repeat with next sample to obtain triplicates.