{"action": "harvest", "output": "", "device": ["centrifugation"], "force": ["<<<2000 RPM>>>"], "time": ["5 min"]}
{"action": "homogenize", "output": "", "reagent": ["<<<cell lysates>>>"]}
{"action": "centrifuge", "output": "", "force": ["2500 RPM"], "time": ["<<<5 min>>>"]}
{"action": "transfer", "output": "", "container": ["fresh centrifuge tubes"]}
{"action": "add", "output": "residual insoluble proteins", "container": ["each sample"], "reagent": [""]}
{"action": "spin", "output": "Hypotonic Buffer", "device": ["ultracentrifugation"], "force": ["<<<55000 RPM>>>"], "reagent": ["residual insoluble proteins"], "time": [""]}
{"action": "transfer", "output": "", "reagent": ["<<<supernatants>>>"], "container": ["fresh centrifuge tubes"]}
{"action": "rinse", "output": "use", "reagent": ["Hypotonic Buffer"]}
{"action": "take", "output": "Hypotonic Buffer", "volume": ["<<<cell lysates>>>"]}
{"action": "dilute", "output": "antibody", "reagent": ["Hypotonic Buffer"]}
{"action": "add", "output": "polyclonal anti-MEKK1", "reagent": ["antibody", "Protein A beads"]}
{"action": "add", "output": "", "reagent": ["polyclonal anti-MEKK1"], "container": ["the lysates"]}
{"action": "rinse", "output": "", "volume": [""]}
{"action": "perform", "output": "Hypotonic Buffer", "device": [""]}
{"action": "rinse", "output": "", "reagent": ["Hypotonic Buffer"]}
{"action": "use", "output": "M", "reagent": ["", ""]}
{"action": "incubate", "output": "", "reagent": ["M"], "container": ["<<<Kinase Assay Buffer>>>"], "temperature": ["<<<30 \u00b0C>>>"], "concentration": [""]}