{"action": "extract", "container": [""], "output": "total RNA", "reagent": ["TRIZOL reagent"], "volume": ["at least 2 x 10^6 cells"]}
{"action": "reverse-transcribe", "output": "cDNA", "reagent": ["total RNA"]}
{"action": "prepare", "output": "cDNA", "reagent": ["2X Sybr Green PCR Master Mix", "cDNA", "primer mix"]}
{"action": "perform", "device": ["Real-Time PCR"], "output": "", "reagent": ["cDNA"], "volume": ["10 ng"]}
{"action": "conduct", "output": ""}
{"action": "analyze", "device": ["built-in SDS Analysis Software"], "output": ""}
{"action": "adjust", "output": ""}