{"action": "sow", "container": ["Surface-sterilized"], "output": "", "temperature": [""]}
{"action": "keep", "output": "", "temperature": ["4 \u00b0C"]}
{"action": "transfer", "container": ["the growth chamber"], "output": "", "temperature": ["22 \u00b0C"]}
{"action": "use", "output": "intracellular pH", "reagent": [""]}
{"action": "place", "output": "", "reagent": ["liquid growth medium"]}
{"action": "monitor", "device": ["confocal microscope"], "output": "F488"}
{"action": "operate", "device": [""], "output": "the calibration curve", "reagent": ["F488", "pH buffer solutions", "the buffer solution"]}
{"action": "recover", "container": ["the external pH buffer solutions"], "output": "", "temperature": [""], "time": ["about 10 min"], "volume": [""]}
{"action": "make", "concentration": [""], "device": ["fluorescence"], "output": "F405-to-[F488] ratio"}
{"action": "make", "output": "", "volume": [""]}
{"action": "confirm", "device": ["the Microsoft Excel"], "output": "in situ titration", "reagent": ["the calibration curve"]}
{"action": "calibrate", "output": ""}