{"action": "collect", "output": "", "volume": ["200 ml"], "container": ["culture medium"]}
{"action": "centrifuge", "output": "crude exosome", "speed": ["<<<1,000 g>>>"], "temperature": ["<<<4\u00b0C>>>"], "reagent": ["intact cells"], "time": ["<<<10 min>>>"]}
{"action": "prepare", "output": "", "concentration": ["10-90% sucrose solutions"], "reagent": ["<<<crude exosome>>>"]}
{"action": "collect", "output": "", "volume": ["<<<2 ml fractions>>>"]}
{"action": "add", "output": "", "volume": ["<<<9 ml PBS>>>"], "mass": [""]}
{"action": "centrifuge", "output": "", "speed": ["<<<100,000 g>>>"], "time": ["<<<1 hr,>>>"], "volume": ["50 \u03bcl"]}
{"action": "include", "output": "exosomes", "reagent": [""]}