{"action": "wash", "output": "", "temperature": ["ice cold"], "force": ["<<<300 x g>>>"], "reagent": ["<<<PBS>>>"], "time": ["3 min"]}
{"action": "transfer", "output": "the clear supernatant", "container": ["<<<new microcentrifuge tube>>>"]}
{"action": "incubate", "output": "", "temperature": ["<<<4 \u00b0C>>>"]}
{"action": "add", "output": "", "volume": ["30 \u00b5l"], "reagent": ["protein G sepharose beads (50% [v/] in PBS)"]}
{"action": "incubate", "output": "the immunoprecipitates", "time": ["2-4 h"], "temperature": ["<<<4 \u00b0C>>>"]}
{"action": "wash", "output": "the supernatant", "reagent": ["<<<the immunoprecipitates>>>"]}
{"action": "wash", "output": "the supernatant", "reagent": ["the immunoprecipitates"]}
{"action": "stop", "output": "the reaction", "reagent": ["SDS-PAGE loading buffer"]}
{"action": "cover", "output": "radioactive contamination", "temperature": ["<<<95 \u00b0C>>>"], "time": ["10 min"]}
{"action": "open", "output": "", "device": ["exposure cassette"], "concentration": [""]}
{"action": "place", "output": "", "container": ["the wrapped gel", "the cassette"]}
{"action": "place", "output": "", "device": ["the phosphor-storage screen"]}
{"action": "place", "output": "", "time": ["30 min"], "temperature": ["<<<room temperature>>>"]}
{"action": "take", "output": "direct light", "device": ["the phosphor-storage screen"], "volume": [""]}
{"action": "detect", "output": "the screen", "device": ["the Typhoon Trio+ system"]}
{"action": "advise", "output": "recombinant PKC-\u03b8", "device": ["", ""]}
{"action": "activate", "output": "", "reagent": ["T538", "recombinant PKC-\u03b8", "direct light"]}
{"action": "conduct", "output": "the kinase assay", "device": [""]}
{"action": "add", "output": "the kinase assay", "reagent": ["ATP-competitive PKC-\u03b8 inhibitor"]}