{"action": "clarify", "device": [""], "output": ""}
{"action": "incubate", "concentration": ["5% CO2"], "output": "", "temperature": ["37 \u00b0C"], "time": ["24 h"]}
{"action": "maintain", "output": "no HSV1-TK", "temperature": ["37 \u00b0C"], "time": ["3 h"]}
{"action": "keep", "output": "control", "reagent": ["no HSV1-TK"], "temperature": [""]}
{"action": "remove", "container": ["the medium"], "output": ""}
{"action": "rinse", "output": "", "reagent": ["PBS"], "temperature": ["ice-cold"], "time": [""]}
{"action": "drain", "output": "the remaining total activities", "time": [""]}
{"action": "add", "container": ["each well"], "output": "", "reagent": ["0.1 N NaOH"]}
{"action": "leave", "output": "cell lysate", "temperature": ["room temperature"]}
{"action": "add", "output": "", "reagent": ["1x Bradford protein assay reagent", "cell lysate"], "volume": ["1 mL", "20 \u03bcL"]}
{"action": "read", "device": ["a spectrophotometer"], "length": [""], "output": "the absorbance"}
{"action": "use", "output": "", "reagent": ["cell lysate"]}
{"action": "transfer", "container": ["a scintillation vial"], "output": "3H-Penciclovir", "volume": ["the remaining cell lysate (480 \u03bcL)"]}
{"action": "provide", "output": "a negative control", "reagent": ["3H-Penciclovir"]}
{"action": "passage", "container": ["the pooled medium"], "length": [""], "output": "3H-Penciclovir", "reagent": ["each sample"]}
{"action": "evaluate", "output": "different samples", "reagent": ["3H-Penciclovir"]}
{"action": "passage", "output": "an average reading/min", "reagent": ["different samples"]}
{"action": "normalize", "concentration": ["protein concentration"], "output": "3H-PCV"}
{"action": "confirm", "output": "3H-PCV", "reagent": ["3H-PCV"]}
{"action": "keep", "output": "protein/total", "reagent": ["3H-PCV"], "temperature": [""]}