Rounding of infected cells, as compared with non-infected cells.\

Purified viral nucleic acid was used as assay control.
{"action": "use", "output": "", "reagent": ["purified viral nucleic acid"]}
RNA integrity was analyzed by agarose gel electrophoresis . #####%%%%% output "agarose gel electrophoresis" are used in line10
{"action": "examine", "output": "agarose gel electrophoresis", "reagent": ["RNA integrity"]}
then stored at -70 °C until use
{"action": "store", "output": "", "temperature": ["-70 °C"]}
The assay was run in duplicate . #####%%%%% output "duplicate" are not used
{"action": "run", "output": "duplicate", "reagent": ["assay"]}
triplicate for each unknown sample
{"action": "duplicate", "output": "", "reagent": ["each unknown sample"]}