Resolve samples on 2% agarose gels using standard electrophoresis methods .
{"action": "resolve", "output": "", "device": ["standard electrophoresis methods"]}
Large wells are required to accommodate each 100 µL sample in a single well.
{"action": "allow", "output": "", "volume": ["each 100 \u00b5L sample"]}
Using a UV transilluminator set to low intensity, excise the target band (~130 bp) from each lane, .
{"action": "excise", "output": "", "device": ["UV transilluminator"]}
Gel slices are held overnight at 4°C,
{"action": "hold", "output": "", "temperature": ["4\u00b0C"]}
the eluent collected the following morning.
{"action": "collect", "output": "", "time": ["the following morning"]}
Collect a small aliquot (~2-10 µL) from each preparation .
{"action": "collect", "output": "", "container": ["each preparation"], "volume": ["~2-10 µL"]}
combine in a multiplex pool for sequencing
{"action": "combine", "output": "", "container": ["a multiplex pool for sequencing"]}
Remaining preparations can be held for 6 months at -20°C .
{"action": "hold", "output": "", "temperature": ["-20\u00b0C"]}
sampled again for sequencing if additional coverage is required for any samples
{"action": "sample", "output": ""}