{"action": "prepare", "output": "recipes 1", "reagent": ["reagents"]}
{"action": "note", "output": "", "reagent": ["DEPC water"], "device": [""]}
{"action": "collect", "output": "", "reagent": ["cells"], "device": ["centrifuge"]}
{"action": "spin", "output": "AE buffer", "time": ["15 sec"], "reagent": ["cells"], "volume": ["1 mL water"]}
{"action": "suspend", "output": "", "reagent": ["AE buffer"], "volume": ["400 \u00b5L"], "temperature": ["room temperature"]}
{"action": "add", "output": "", "reagent": ["10% SDS"], "volume": ["<<<40 \u00b5L>>>"]}
{"action": "add", "output": "", "reagent": ["hot phenol/AE"], "volume": ["500 \u00b5L"], "temperature": ["65 \u00b0C"]}
{"action": "sort", "output": "", "reagent": ["phenol/AE"], "time": ["1 min"]}
{"action": "incubate", "output": "", "temperature": ["65 \u00b0C"], "time": ["<<<5 min>>>"]}
{"action": "suspend", "output": "", "reagent": ["liquid nitrogen"]}
{"action": "see", "output": "", "reagent": ["<<<cells>>>"], "temperature": ["30 \u00b0C"]}
{"action": "transfer", "output": "supernatant", "volume": ["400 \u03bcL"]}
{"action": "add", "output": "", "volume": ["400 \u00b5L"], "reagent": ["phenol: CHCl3/AE-Na"]}
{"action": "spin", "output": "CHCl3", "force": ["20,000 x g"], "time": ["5 min"], "device": ["a standard laboratory microfuge"], "reagent": ["supernatant"]}
{"action": "add", "output": "", "reagent": ["CHCl3"], "container": ["isoamyl alcohol (24:1)"]}
{"action": "transfer", "output": "aqueous supernatant", "container": ["a fresh 1.5 mL microfuge tube"]}
{"action": "repeat", "output": "", "container": ["the aqueous phase"], "reagent": ["<<<aqueous supernatant>>>"]}
{"action": "add", "output": "", "reagent": ["3 M NaOAc (pH 5)"], "volume": ["1/10 volume"]}
{"action": "add", "output": "", "volume": ["<<<ethanol>>>"]}
{"action": "place", "output": "", "time": ["at least 30 min"], "temperature": ["<<<-20 \u00b0C>>>"]}
{"action": "spin", "output": "", "device": ["<<<the microfuge>>>"], "force": ["20,000 x g"], "time": ["15 min"], "temperature": ["<<<4 \u00b0C>>>"]}
{"action": "add", "output": "the liquid droplets", "reagent": ["<<<ice-cold 75% EtOH>>>"], "time": ["around 10 min"], "temperature": ["4 \u00b0C"]}
{"action": "pour", "output": "", "reagent": ["the liquid droplets"], "container": ["<<<the tube>>>"]}
{"action": "add", "output": "RNA", "volume": ["30-50 \u00b5L"], "container": ["\u00b5L ddH2O (DEPC)"]}
{"action": "dissolve", "output": "", "reagent": ["<<<RNA>>>"], "time": ["30 min"], "temperature": ["<<<37 \u00b0C>>>"]}