{"action": "prepare", "output": "inhibitor compound", "concentration": ["50 \u00b5M RNA/DNA oligos"], "reagent": ["DNase/[RNase-free] water"], "volume": ["50 \u00b5l"], "container": ["PCR tube"]}
{"action": "dissolve", "output": "water", "reagent": ["inhibitor compound"]}
{"action": "add", "output": "", "reagent": ["<<<water>>>"], "container": ["<<<96-well plate>>>"]}
{"action": "add", "output": "", "volume": ["80 \u00b5l RT reaction mix (1.25x)"]}
{"action": "add", "output": "EDTA", "volume": ["10 \u00b5l inhibitor dilution"], "container": ["samples"]}
{"action": "stop", "output": "", "reagent": ["<<<EDTA>>>"], "volume": ["<<<50 \u00b5l>>>"]}
{"action": "quantify", "output": "490/528 nm", "device": ["Victor 3"]}
{"action": "subtract", "output": "blank value", "reagent": ["490/528 nm"]}
{"action": "calculate", "output": "signal", "concentration": ["IC50 value"]}