Weigh 10 g fresh silage, .
{"action": "weigh", "output": "", "reagent": ["fresh silage"], "mass": ["10 g"]}
transfer to glass jar blender
{"action": "transfer", "output": "", "container": ["glass jar blender"]}
Add 100 mL Milli-Q water to blender.
{"action": "add", "output": "", "volume": ["100 mL"], "reagent": ["Milli-Q water"]}
Blend for 30s at intermediate rate.
{"action": "blend", "output": "", "time": ["30s"]}
Filter mixture using PYREX Gooch Type Filtering Crucible vacuum filtration equipment, keep liquid filtrate.
{"action": "filter", "output": "", "device": ["PYREX Gooch Type Filtering Crucible vacuum filtration equipment"]}
Recover liquid phase using a plastic syringe, filter through 0.45 μm PES membrane, . #####%%%%% output "liquid phase" are used in line22
{"action": "recover", "output": "liquid phase", "device": ["plastic syringe"]}
Prepare calibration curves for lactic acid .
{"action": "prepare", "output": "", "reagent": ["lactic acid"]}
Analyze calibration curves using RP-HPLC-DAD.
{"action": "analyze", "output": "", "device": ["RP-HPLC-DAD"]}
integrate chromatographic peaks #####%%%%% output "chromatographic peaks" are not used
{"action": "embed", "output": "chromatographic peaks", "reagent": [""]}
Place 500 μL extract in chromatographic vial, analyze using RP-HPLC-DAD.
{"action": "analyze", "output": "", "device": ["RP-HPLC-DAD"]}
integrate chromatographic peaks #####%%%%% output "chromatographic peaks" are not used
{"action": "integrate", "output": "chromatographic peaks", "reagent": ["liquid phase"]}