{"action": "mix", "container": ["conical centrifuge tube"], "output": "Ficoll-Hypaque", "reagent": ["fresh EDTA blood", "room-temperature PBS"], "temperature": ["room temperature"]}
{"action": "layer", "container": ["the blood/PBS mixture"], "output": "", "reagent": ["Ficoll-Hypaque"], "volume": ["3 ml"]}
{"action": "centrifuge", "force": ["900 x g"], "output": "", "temperature": ["room temperature"], "time": ["30 minutes"]}
{"action": "remove", "device": [""], "output": "the upper plasma layer"}
{"action": "transfer", "container": ["the mononuclear cell layer"], "output": ""}
{"action": "rinse", "force": ["400 x g"], "output": "", "reagent": ["PBS"], "time": ["10 minutes"], "times": ["three times"], "volume": ["3x volume of the cell layer"]}
{"action": "resuspend", "container": ["the cells"], "output": "", "reagent": [""], "volume": [""]}