{"action": "pass", "output": "the cells", "device": ["the nylon strainer"], "reagent": ["<<<medium>>>"]}
{"action": "detect", "output": "FITC-conjugated anti-CD4 CD25", "reagent": ["PBS/BSA"]}
{"action": "incubate", "output": "anti-FITC microbeads", "reagent": ["FITC-conjugated anti-CD4 CD25"], "temperature": ["ice"], "time": [""]}
{"action": "resuspend", "output": "", "reagent": ["<<<cells>>>"], "volume": ["<<<2 ml>>>"], "solution": ["PBS/BSA"]}
{"action": "incubate", "output": "release reagent", "reagent": ["anti-FITC microbeads"], "temperature": ["the refrigerator"], "time": [""]}
{"action": "purify", "output": "", "device": ["<<<Auto MACS sorter>>>"]}
{"action": "release", "output": "CD4+CD25+ Tregs", "reagent": ["release reagent (available"], "device": ["the multisort kit"]}
{"action": "incubate", "output": "the purified CD4T cell suspension", "reagent": ["\u03b1-biotin microbeads"], "volume": ["<<<500 \u00b5l>>>"]}
{"action": "recover", "output": "CD4+CD25&#x2013;", "reagent": ["CD25cells"]}
{"action": "add", "output": "", "reagent": ["3\u00d710^4 irradiated (3000 Rad) T cell-depleted splenocytes"]}
{"action": "use", "output": "CFSE+ responders", "reagent": ["Treg Tcon cells", "responders"]}
{"action": "perform", "output": "forward scatter", "reagent": ["CFSE+ responders"]}
{"action": "evaluate", "output": "cell death", "device": ["cytometry analyses"], "reagent": ["CD4+CD25&#x2013;"]}