{"action": "cut", "output": ""}
{"action": "remove", "output": "PBS", "container": ["the mesentery"], "reagent": [""], "device": [""]}
{"action": "rinse", "output": "", "reagent": ["<<<PBS>>>"]}
{"action": "cut", "output": "", "device": ["a scissor"]}
{"action": "cut", "output": "", "length": ["5 \u03bcm"], "device": ["Leica CM3050 cryostat"]}
{"action": "fix", "output": "", "time": ["<<<seven minutes>>>"], "temperature": ["ice-cold acetone"]}
{"action": "store", "output": "", "temperature": ["<<<-80\u00b0C>>>"], "container": ["desiccant bags"]}
{"action": "handle", "output": "staining", "device": ["a Dako Pen"], "reagent": [""]}
{"action": "block", "output": "TBS-T", "reagent": ["antibodies", "TBS-T"]}
{"action": "dip", "output": "", "reagent": ["<<<TBS-T>>>"]}
{"action": "apply", "output": "the staining solutions", "reagent": ["<<<TBS-T>>>"], "time": ["45 minutes"]}
{"action": "rinse", "output": "DAPI nucleic acid stain", "reagent": ["the staining solutions"]}
{"action": "stain", "output": "PBS", "reagent": ["DAPI nucleic acid stain"], "time": ["<<<30 seconds>>>"]}
{"action": "rinse", "output": "", "reagent": ["PBS"], "device": ["slide-beakers"]}
{"action": "acquire", "output": "", "device": ["Leica DMRA2 fluorescence microscope"]}
{"action": "keep", "output": ""}
{"action": "apply", "output": "images", "device": [""]}
{"action": "estimate", "output": "small intestinal LP IgA+ plasma cells", "device": ["ImageJ"]}
{"action": "quantify", "output": "five separate images", "reagent": ["images"]}