{"action": "amplify", "output": "", "reagent": ["the barcoded insert"]}
{"action": "go", "output": "the Qiagen\u00ae PCR Purification Kit", "reagent": [""]}
{"action": "pool", "output": "Lentiviral Plasmid", "reagent": ["the Qiagen\u00ae PCR Purification Kit"]}
{"action": "digest", "output": "digested products", "reagent": ["Lentiviral Plasmid", "XmaI", "Lentiviral Plasmid"]}
{"action": "run", "output": "Qiagen\u00ae MinElute Gel Extraction Kit (Qiagen, 28604)", "reagent": ["<<<digested products>>>"]}
{"action": "extract", "output": "H20", "reagent": ["Qiagen\u00ae MinElute Gel Extraction Kit (Qiagen, 28604)"]}
{"action": "elute", "output": "Lentiviral Plasmid", "reagent": ["H20"], "volume": ["25 l"]}
{"action": "ligate", "output": "", "reagent": ["barcoded insert", "Lentiviral Plasmid", "Ligation Buffer"]}
{"action": "read", "output": "the ligation product", "time": [""]}
{"action": "purify", "output": "", "reagent": ["the ligation product"], "device": ["the Qiagen\u00ae PCR Purification Kit (Qiagen, 28106) 8"]}
{"action": "transform", "output": "", "reagent": ["Thermo Fisher, 18290015", "ElectroMAX DH10B cells"]}
{"action": "provide", "output": "colonies", "reagent": [""]}
{"action": "peel", "output": "", "reagent": ["bacterial colonies", "LB-Amp", ""], "container": ["<<<plate>>>"]}
{"action": "determine", "output": "Sanger", "concentration": ["plasmid concentration"]}
{"action": "confirm", "output": "", "reagent": ["Sanger"]}