{"in": [], "out": "fresh blood specimens"}
{"in": ["Lymphoprep density gradient medium", "PBMC"], "out": "MACS buffer"}
{"in": ["total CD4<sup>+</sup> T cells"], "out": ""}
{"in": ["MACS buffer"], "out": ""}
{"in": ["CD4<sup>+</sup> T cells (negative fraction)"], "out": ""}
{"in": ["Treg cells"], "out": ""}
{"in": [], "out": ""}
{"in": ["culture medium"], "out": "the resulting Treg cells"}
{"in": ["the resulting Treg cells"], "out": "CFSE"}
{"in": ["CFSE"], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": "percent inhibition"}