{"in": ["the barcoded insert"], "out": ""}
{"in": [""], "out": "the Qiagen\u00ae PCR Purification Kit"}
{"in": ["the Qiagen\u00ae PCR Purification Kit"], "out": "Lentiviral Plasmid"}
{"in": ["Lentiviral Plasmid", "Lentiviral Plasmid", "XmaI"], "out": "digested products"}
{"in": ["digested products"], "out": "Qiagen\u00ae MinElute Gel Extraction Kit (Qiagen, 28604)"}
{"in": ["Qiagen\u00ae MinElute Gel Extraction Kit (Qiagen, 28604)"], "out": "H20"}
{"in": ["H20"], "out": "Lentiviral Plasmid"}
{"in": ["Lentiviral Plasmid", "Ligation Buffer", "barcoded insert"], "out": ""}
{"in": [], "out": "the ligation product"}
{"in": ["the ligation product"], "out": ""}
{"in": ["ElectroMAX DH10B cells", "Thermo Fisher, 18290015"], "out": ""}
{"in": [""], "out": "colonies"}
{"in": ["", "LB-Amp", "bacterial colonies"], "out": ""}
{"in": [], "out": "Sanger"}
{"in": ["Sanger"], "out": ""}