{"action": "make", "length": [""], "output": "", "reagent": ["double-stranded", "salmon sperm DNA 1"]}
{"action": "add", "container": ["96-well"], "output": "", "volume": ["\u03bcL"]}
{"action": "wrap", "length": [""], "output": "", "reagent": [""]}
{"action": "incubate", "output": "the coating antibody solution", "temperature": ["4 \u00b0C"]}
{"action": "remove", "output": "1x PBS-Tween", "reagent": ["the coating antibody solution"]}
{"action": "wash", "output": "", "reagent": ["1x PBS-Tween"]}
{"action": "rinse", "container": ["the plate"], "output": ""}
{"action": "add", "container": ["well"], "output": ""}
{"action": "incubate", "container": ["the plate"], "output": "the blocking solution", "temperature": ["room temperature (RT)"], "time": [""]}
{"action": "remove", "output": "1x PBS-Tween", "reagent": ["the blocking solution"]}
{"action": "wash", "output": "", "reagent": ["1x PBS-Tween"]}
{"action": "rinse", "container": ["the plate"], "output": ""}
{"action": "keep", "output": "the spleen", "temperature": ["4 \u00b0C"]}
{"action": "export", "output": "spleen pieces", "reagent": ["the spleen", "the spleen"], "time": [""]}
{"action": "create", "output": "a single-cell suspension", "reagent": ["spleen pieces"]}
{"action": "transfer", "container": ["the cells"], "output": ""}
{"action": "turn", "output": "", "temperature": ["4 \u00b0"]}
{"action": "suspend", "concentration": ["0.17 M ammonium chloride"], "container": ["the cells"], "output": ""}
{"action": "keep", "output": "", "time": ["5 min"]}
{"action": "remove", "output": "the supernatant", "reagent": ["a single-cell suspension"]}
{"action": "suspend", "output": "", "reagent": ["DMEM", "the supernatant"]}
{"action": "estimate", "output": "the cells", "reagent": [""]}
{"action": "replace", "concentration": ["10%"], "output": ""}
{"action": "add", "container": ["DNA-coated"], "output": "", "volume": ["50"]}
{"action": "incubate", "output": "", "temperature": ["30 \u00b0C"], "time": ["2 h"]}
{"action": "add", "container": ["the cells"], "output": "", "volume": ["50"]}
{"action": "centrifuge", "container": ["the cells"], "force": ["300 RCF"], "output": "CO2", "reagent": [""], "temperature": ["4 \u00b0C"], "time": [""]}
{"action": "incubate", "output": "", "reagent": ["CO2"], "temperature": [""], "time": [""]}
{"action": "remove", "container": ["the cells"], "output": "", "volume": [""]}
{"action": "wash", "output": "", "reagent": ["the cells"], "time": [""]}
{"action": "rinse", "container": ["the plates"], "output": "streptavidin alkaline phosphatase (1:1,000 [in 1]% BSA/PBS)"}
{"action": "add", "container": ["the plate"], "output": "", "reagent": ["streptavidin alkaline phosphatase 1:1,000 [in 1]% BSA/PBS"]}
{"action": "incubate", "container": ["the plate"], "output": "10x PBS-Tween 20", "temperature": ["RT"], "time": ["1 h"]}
{"action": "wash", "output": "", "reagent": ["10x PBS-Tween 20"]}
{"action": "rinse", "container": ["the plate"], "output": "AMP buffer"}
{"action": "add", "output": "the plate", "reagent": ["AMP buffer"]}