{"action": "develop", "device": [""], "output": "", "reagent": ["", ""]}
{"action": "microwave", "output": "", "reagent": ["", ""], "time": [""]}
{"action": "allow", "output": "", "temperature": ["37\u00b0C"], "time": ["seven days"]}
{"action": "continue", "output": "E coli O127, B8", "temperature": ["37\u00b0C"], "time": ["seven more days"]}
{"action": "collect", "output": "floating microglia", "reagent": ["astrocytes"]}
{"action": "treat", "concentration": [""], "output": "media 0.5ml TriZol", "reagent": ["E coli O127, B8"], "time": ["6h"]}
{"action": "do", "output": "RNA extractionwere", "reagent": ["media 0.5ml TriZol"]}
{"action": "examine", "device": ["light microscopy"], "output": ""}
{"action": "plate", "container": ["floating cells"], "device": [""], "output": "RNAseq"}
{"action": "separate", "output": "TRIzol Reagent (Life Technologies)", "reagent": ["RNAseq"]}
{"action": "extract", "output": "a RNA Nano Chip (Agilent RNA 6000 Nano Chips)", "reagent": ["TRIzol Reagent (Life Technologies)", "cultured microglia"]}
{"action": "establish", "device": ["Agilent 2100 BioAnalyzer"], "output": "Illumina TruSeq RNA Sample Preparation v2 kit (Illumina)", "reagent": ["a RNA Nano Chip (Agilent RNA 6000 Nano Chips)"]}
{"action": "prepare", "output": "", "reagent": ["Illumina TruSeq RNA Sample Preparation v2 kit (Illumina)"]}
{"action": "perform", "device": ["BioAnalyzer"], "output": ""}
{"action": "perform", "device": ["an Illumina HiSeq2500"], "output": "Single-end"}