{"action": "centrifuge", "force": ["200 x g"], "output": "", "temperature": ["room temperature"], "time": ["5 min"]}
{"action": "remove", "output": "the supernatant", "reagent": [""]}
{"action": "suspend", "output": "", "reagent": ["ACK lysing buffer"], "time": ["1 min"], "volume": ["2 ml"]}
{"action": "repeat", "output": "", "reagent": ["the ACK"]}
{"action": "rinse", "container": ["a 40 \u03bcm nylon strainer", "the cell suspension"], "device": [""], "output": "", "reagent": ["the supernatant"]}
{"action": "wash", "container": ["the strainer"], "output": "", "reagent": ["2 ml 1x DPBS"], "time": ["5 min"]}
{"action": "wash", "container": ["a 100 mm Petri dish"], "output": "", "reagent": ["1x DPBS with 20 ng/ml murine M-CSF"]}
{"action": "suspend", "output": "", "reagent": ["complete DMEM medium"], "volume": ["15 ml"]}
{"action": "incubate", "output": "fresh complete DMEM medium", "temperature": ["37 \u00b0C"]}
{"action": "replace", "output": "", "reagent": ["fresh complete DMEM medium"]}
{"action": "repeat", "output": "", "reagent": ["fresh complete DMEM medium"]}