{"in": ["PBS"], "out": ""}
{"in": [], "out": "the clear supernatant"}
{"in": [], "out": ""}
{"in": ["protein G sepharose beads (50% [v/] in PBS)"], "out": ""}
{"in": [], "out": "the immunoprecipitates"}
{"in": ["the immunoprecipitates"], "out": "the supernatant"}
{"in": ["the immunoprecipitates"], "out": "the supernatant"}
{"in": ["SDS-PAGE loading buffer"], "out": "the reaction"}
{"in": [], "out": "radioactive contamination"}
{"in": [], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": "direct light"}
{"in": [], "out": "the screen"}
{"in": [], "out": "recombinant PKC-\u03b8"}
{"in": ["T538", "direct light", "recombinant PKC-\u03b8"], "out": ""}
{"in": [], "out": "the kinase assay"}
{"in": ["ATP-competitive PKC-\u03b8 inhibitor"], "out": "the kinase assay"}