{"action": "set", "output": "primers", "reagent": ["10 mM Tris-HCl", "template DNA (82.5 ng)"], "volume": ["20 \u00b5l"]}
{"action": "run", "output": "", "reagent": [""], "temperature": ["60\u00b0C", "72\u00b0C", "94\u00b0C", "94\u00b0C"], "time": ["1 min", "1 min", "1.75 min", "2 min"]}
{"action": "detect", "output": "germline alleles", "reagent": ["DH-JH recombination", "DHL5\u2019", "primers"]}
{"action": "detect", "output": "", "reagent": ["germline alleles", "primers"]}