{"action": "develop", "output": "", "reagent": ["", ""], "device": [""]}
{"action": "microwave", "output": "", "reagent": ["", ""], "time": [""]}
{"action": "allow", "output": "", "time": ["seven days"], "temperature": ["<<<37\u00b0C>>>"]}
{"action": "continue", "output": "E coli O127, B8", "time": ["seven more days"], "temperature": ["37\u00b0C"]}
{"action": "collect", "output": "floating microglia", "reagent": ["<<<astrocytes>>>"]}
{"action": "treat", "output": "media 0.5ml TriZol", "reagent": ["E coli O127, B8"], "time": ["<<<6h>>>"], "concentration": [""]}
{"action": "do", "output": "RNA extractionwere", "reagent": ["<<<media 0.5ml TriZol>>>"]}
{"action": "examine", "output": "", "device": ["<<<light microscopy>>>"]}
{"action": "plate", "output": "RNAseq", "container": ["<<<floating cells>>>"], "device": [""]}
{"action": "separate", "output": "TRIzol Reagent (Life Technologies)", "reagent": ["RNAseq"]}
{"action": "extract", "output": "a RNA Nano Chip (Agilent RNA 6000 Nano Chips)", "reagent": ["cultured microglia", "TRIzol Reagent (Life Technologies)"]}
{"action": "establish", "output": "Illumina TruSeq RNA Sample Preparation v2 kit (Illumina)", "reagent": ["a RNA Nano Chip (Agilent RNA 6000 Nano Chips)"], "device": ["Agilent 2100 BioAnalyzer"]}
{"action": "prepare", "output": "", "reagent": ["Illumina TruSeq RNA Sample Preparation v2 kit (Illumina)"]}
{"action": "perform", "output": "", "device": ["BioAnalyzer"]}
{"action": "perform", "output": "Single-end", "device": ["an Illumina HiSeq2500"]}