{"action": "grow", "output": "pellet", "reagent": ["the strains"]}
{"action": "suspend", "output": "cells", "reagent": ["pellet"]}
{"action": "transfer", "output": "", "reagent": ["<<<cells>>>"], "container": ["glass beads"], "temperature": ["\u201320 \u00b0C"]}
{"action": "grab", "output": "", "reagent": ["<<<ethanol dry ice bath>>>"]}
{"action": "hold", "output": "", "device": [""]}
{"action": "add", "output": "", "volume": ["300 mL phenol:chloroform (pH 4.7)"], "container": ["the samples"]}
{"action": "vortex", "output": "", "device": ["eppendorf tubes"], "temperature": ["ice"]}
{"action": "transfer", "output": "the top layer (aqueous phase)", "container": ["new tubes"]}
{"action": "repeat", "output": "the RNA", "reagent": ["phenol extraction", "phenol:chloroform (pH 4.7)"], "time": ["15 minutes"], "temperature": ["4 \u00b0C"]}
{"action": "precipitate", "output": "the RNA pellet", "reagent": ["the RNA", "cold 100% ethanol"], "time": ["25 minutes"], "temperature": ["4 \u00b0C"]}
{"action": "suspend", "output": "", "reagent": ["the RNA pellet", "cold sodium acetate (0.3 M, pH 4.5)", "the top layer (aqueous phase)"], "volume": ["60 \u00b5L"], "concentration": [""]}
{"action": "precipitate", "output": "", "volume": ["400 \u00b5L 100% ethanol"], "temperature": ["<<<4 \u00b0C>>>"]}
{"action": "dry", "output": "the pellet", "temperature": ["ice"], "device": [""]}
{"action": "resuspend", "output": "the RNA", "container": ["the pellet"], "volume": ["50 \u00b5L"], "reagent": ["<<<the pellet>>>"]}
{"action": "evaluate", "output": "spectrophotometry (A260)", "reagent": ["<<<the RNA>>>"]}