The following protocol describes a noninvasive, method to deliver materials of interest to mouse lungs. #####%%%%% output "ketamine" are used in line6
{"action": "define", "output": "ketamine", "reagent": ["interest"]}
This procedure was approved by the National Cancer Institute Animal Care Committee.

Anesthesia First, anesthetize the mouse using a mixture of ketamine (100 mg/kg body weight .
{"action": "anesthetize", "output": "", "reagent": ["ketamine"]}
Apply vet ointment to the eyes of the mouse in order to prevent drying of the eyes during anesthesia.
{"action": "apply", "output": "", "reagent": ["vet ointment"]}
After several minutes, pinch a foot of the mouse to check for consciousness.
{"action": "check", "output": "", "reagent": ["the mouse"]}
Once confirmed unconscious, place the mouse on an intubation stand angled at ~60° .
{"action": "place", "output": "", "device": ["an intubation stand"]}
Intratracheal Intubation Gently retract the mouse's tongue to one side using a Q-tip.
{"action": "retract", "output": "", "length": ["one side"], "device": ["a Q-tip"]}
When using the BioLITE Intubation Illumination System, carefully insert the intubation system until the larynx is visualized with the aid of the fiber-optic light guide.
{"action": "insert", "output": "", "device": ["the BioLITE Intubation Illumination System", "the intubation system", "fiber-optic"], "length": [""]}
insert the fiber over the epiglottis
{"action": "insert", "output": "", "device": ["the fiber"], "container": ["the epiglottis"]}
Note: In order to obtain the proper length of catheter to be inserted, initially measure the length between the mouth by using a practice mouse of a similar size in advance (Figure 1).
{"action": "note", "output": "", "device": ["a practice mouse"]}
The length largely depends on the size of the mouse.

Insertion of the catheter should stop above the bifurcation point (~1.5 cm for mouse with ~25 g of body weight).
{"action": "stop", "output": "", "device": ["the catheter"]}
This ensures that the intubated material will go to all lobes.

At least 50 practice mice may be required for a person performing the intubation to become proficient in the technique (Proficient means that the success rate of intubation is more than 95%).

Once the catheter is inserted, quickly remove the fiber-optic light guide from the catheter to allow the animal to breathe normally.
{"action": "remove", "output": "", "device": ["the catheter"]}
When the Intubation Illumination System is not used, directly insert a catheter as described.
{"action": "insert", "output": "", "device": ["a catheter"], "container": ["the larynx"]}
Add a solution containing materials of interest to a catheter.
{"action": "add", "output": "", "device": ["a catheter"], "reagent": ["a solution containing materials of interest"]}
Make sure that the solution is sucked into the lung immediately after addition.
{"action": "suck", "output": "", "reagent": ["the solution"], "container": ["the lung"]}
Fifty microliters for ~25 g mouse body weight is routinely used.

Animal Recovery As soon as the solution is sucked into the lung, take down the mouse from the stand, .
{"action": "take", "output": "", "device": ["the mouse"], "container": ["the stand"]}
put it back into the original cage
{"action": "put", "output": "", "container": ["the original cage"]}
Observe the mouse until it starts moving.
{"action": "observe", "output": "", "reagent": ["", ""]}
Once confirmed that the mouse is in good condition, return the cage to a rack.
{"action": "return", "output": "", "container": ["the cage"], "device": ["a rack"]}
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