{"action": "transfer", "container": ["a 1.5 ml microfuge tube"], "output": "buffer", "reagent": ["serum"]}
{"action": "add", "output": "the sample/XBP mixture", "reagent": ["buffer"]}
{"action": "add", "output": "buffer XWP", "reagent": ["the sample/XBP mixture"]}
{"action": "add", "output": "", "reagent": ["buffer XWP"], "volume": ["10 ml"]}
{"action": "transfer", "container": ["a fresh collection tube", "the spin column"], "output": "Qiazol", "volume": [""]}
{"action": "add", "output": "", "reagent": ["Qiazol"], "volume": ["700 \u03bcl"]}
{"action": "transfer", "container": ["a PLG tube.<sup>1</sup> 13"], "output": "the flow-though"}
{"action": "incubate", "output": "", "temperature": ["room temperature"], "time": ["5 minutes"]}
{"action": "add", "mass": [""], "output": "", "volume": ["90 \u00b5l chloroform"]}
{"action": "shake", "output": "", "time": ["15 seconds"]}
{"action": "incubate", "output": "", "temperature": ["room temperature"], "time": ["3 min"]}
{"action": "transfer", "container": ["a new microcentrifuge tube"], "output": ""}
{"action": "measure", "container": ["the aqueous phase"], "output": ""}
{"action": "add", "output": "", "reagent": ["100% ethanol"]}
{"action": "assemble", "container": ["a MinElute spin column"], "output": "", "reagent": [""]}
{"action": "load", "device": [""], "output": "", "reagent": ["the flow-though"], "volume": ["up to 700"]}
{"action": "move", "output": "buffer RWT", "time": ["23-25"]}
{"action": "add", "output": "", "reagent": ["buffer RWT"], "volume": ["700 \u00b5l"]}
{"action": "remove", "output": "the flow-through", "volume": [""]}
{"action": "remove", "output": "the flow-through", "volume": [""]}
{"action": "remove", "container": ["the collection tube"], "output": "the flow-through.<sup>4</sup> 36", "reagent": ["the flow-through"], "volume": [""]}
{"action": "transfer", "container": ["a new 2 ml collection tube (supplied)"], "device": ["RNeasy MinElute"], "output": "", "volume": [""]}
{"action": "open", "container": ["the spin column"], "output": ""}
{"action": "centrifuge", "container": ["membrane.<sup>5</sup>"], "output": "", "speed": ["full speed (14,000 xg)"], "time": ["5 min"]}
{"action": "remove", "container": ["the collection tube"], "output": "the flow-through.<sup>4</sup> 39", "reagent": ["the flow-through.<sup>4</sup> 36"], "volume": [""]}
{"action": "transfer", "container": ["a new 1.5 ml collection tube (supplied)"], "device": ["RNeasy MinElute"], "output": "RNase-free water", "volume": [""]}
{"action": "add", "output": "", "reagent": ["RNase-free water"], "volume": ["14 \u00b5l"]}