{"action": "obtain", "output": "fresh blood specimens"}
{"action": "identify", "output": "MACS buffer", "reagent": ["PBMC", "Lymphoprep density gradient medium"]}
{"action": "isolate", "output": "", "reagent": ["total CD4<sup>+</sup> T cells"], "device": ["CD4<sup>+</sup> T cell Isolation Kit"]}
{"action": "prepare", "output": "", "container": ["LS column"], "reagent": ["<<<MACS buffer>>>"], "volume": ["<<<3 ml>>>"]}
{"action": "wash", "output": "", "reagent": ["CD4<sup>+</sup> T cells (negative fraction)"]}
{"action": "treat", "output": "", "reagent": ["<<<Treg cells>>>"], "time": ["<<<24 hs>>>"]}
{"action": "leave", "output": "", "concentration": ["0.5-1.0\u00d710^5 cells per well"]}
{"action": "rinse", "output": "the resulting Treg cells", "reagent": ["<<<culture medium>>>"]}
{"action": "estimate", "output": "CFSE", "container": ["the cells"], "volume": [""], "concentration": [""], "reagent": ["the resulting Treg cells"]}
{"action": "rinse", "output": "", "reagent": ["CFSE"]}
{"action": "maintain", "output": "", "concentration": ["2\u00d710<[sup>3</sup>] anti-CD3/CD28 beads"], "time": ["<<<4 days>>>"], "temperature": [""]}
{"action": "maintain", "output": "", "concentration": ["2\u00d710<[sup>3</sup>] anti-CD3/CD28 beads"], "time": ["4 days"], "temperature": [""]}
{"action": "connect", "output": "percent inhibition", "device": [""]}