{"action": "examine", "output": "", "time": [""]}
{"action": "reset", "output": "graded EtOH series", "time": ["<<<55 min>>>"]}
{"action": "wash", "output": "", "reagent": ["0.5% PBT"], "time": ["10 min"]}
{"action": "use", "output": "graded EtOH series", "device": ["a rocker"], "temperature": ["<<<4 \u030aC>>>"]}
{"action": "rinse", "output": "", "time": ["10 min"], "reagent": ["graded EtOH series"]}
{"action": "operate", "output": "", "reagent": ["5% acetic acid"], "device": ["cold room"], "temperature": ["4 \u030aC"], "time": ["5 min"]}
{"action": "correct", "output": "tissue", "concentration": ["<<<2% PFA>>>"], "temperature": ["25 \u030aC"], "time": ["55 min"]}
{"action": "wash", "output": "", "reagent": ["<<<0.5% PBT>>>"], "time": ["10 min"]}
{"action": "wash", "output": "pre-hybr soln", "temperature": ["4 \u030aC"], "time": ["5 min each"], "reagent": ["<<<PBS>>>"], "force": [""]}
{"action": "prepare", "output": "", "reagent": ["pre-hybr soln"]}
{"action": "grab", "output": "", "device": [""]}
{"action": "prepare", "output": "the pre-hybr soln", "volume": ["~48\u03bcL"]}
{"action": "soak", "output": "the hybridization reaction", "reagent": ["the pre-hybr soln"], "temperature": ["37 \u030aC"]}
{"action": "add", "output": "", "reagent": ["the hybridization reaction"]}
{"action": "incubate", "output": "pre-hybr soln", "time": ["<<<10 min>>>"], "temperature": [""]}
{"action": "wash", "output": "pre-hybr soln", "reagent": ["<<<pre-hybr soln>>>"]}
{"action": "wash", "output": "30% formamide washing soln", "reagent": ["pre-hybr soln"]}
{"action": "wash", "output": "2xSSC washing soln", "reagent": ["30% formamide washing soln"]}
{"action": "wash", "output": "each", "reagent": ["2xSSC washing soln"]}
{"action": "wash", "output": "", "temperature": ["25 \u030aC"], "time": ["10 min each"], "force": [""], "reagent": ["each"]}
{"action": "dehydrate", "output": "BB-SIM", "concentration": ["30%, 50%, 75%, 100%, 100%, 100%, 10 min each"]}
{"action": "hold", "output": "", "reagent": ["BB-SIM"]}