{"action": "add", "output": "5,000-10,000 cpm", "reagent": ["160 mM KMnO4"], "volume": ["40 \u03bcl"]}
{"action": "add", "output": "phenol/chloroform", "reagent": ["500 mM EDTA"], "volume": ["5.3 \u03bcl"]}
{"action": "precipitate", "output": "", "reagent": ["<<<ethanol>>>"]}
{"action": "dissolve", "output": "", "volume": ["70 \u03bcl"]}
{"action": "incubate", "output": "", "temperature": ["<<<90 \u00b0C>>>"], "time": ["<<<30 min>>>"]}
{"action": "repeat", "output": "", "volume": ["30 \u03bcl water"]}
{"action": "dissolve", "output": "", "volume": ["<<<50 \u03bcl water>>>"]}
{"action": "add", "output": "ethanol", "volume": ["4 \u03bcl 300 mM NaOAc,"]}
{"action": "precipitate", "output": "pellets", "reagent": ["<<<ethanol>>>"]}
{"action": "rinse", "output": "non-labeled DNA", "reagent": ["<<<pellets>>>"]}
{"action": "go", "output": "similar steps", "reagent": ["<<<non-labeled DNA>>>"]}
{"action": "add", "output": "primer extension", "reagent": ["5'-32P-labeled primer"]}
{"action": "perform", "output": "", "reagent": ["primer extension"]}
{"action": "separate", "output": "", "device": ["denaturing gel"]}
{"action": "visualize", "output": "autoradiography", "reagent": [""]}