{"action": "replace", "container": ["medium"], "output": "Digest mESCs", "volume": [""]}
{"action": "digest", "container": ["96-well plates"], "output": "mESCs", "reagent": ["0.05% trypsin"]}
{"action": "remove", "container": ["24-well plates"], "output": ""}
{"action": "confirm", "output": "positive colonies", "reagent": ["sgRNAs analysis (SPH primers)"]}
{"action": "replace", "container": ["medium"], "output": "", "volume": [""]}
{"action": "sort", "container": ["96-well plates"], "device": ["FACS"], "output": "single cells"}
{"action": "confirm", "output": "", "reagent": [""]}
{"action": "remove", "container": ["24-well plates"], "output": ""}
{"action": "confirm", "output": "positive colonies", "reagent": ["positive colonies"]}
{"action": "take", "container": ["colonies"], "device": ["confocal microscope"], "output": "fluorescence images"}