{"action": "rinse", "output": "", "reagent": ["MSC"], "volume": ["2 ml PBS"]}
{"action": "trypsinize", "output": "", "volume": ["0.5 ml trypsin"]}
{"action": "transfer", "container": ["15 ml tube"], "output": "FBS"}
{"action": "add", "output": "", "reagent": ["FBS"], "volume": ["10 ml DMEM"]}
{"action": "incubate", "output": "osteogenic differentiation medium", "temperature": ["37 \u00b0C, 5% CO2"]}
{"action": "wash", "output": "", "reagent": ["osteogenic differentiation medium"], "volume": ["2 ml PBS"]}
{"action": "change", "output": "Alizarin red S", "time": ["every 2 days for 10 days"]}
{"action": "rinse", "output": "100 \u03bcl urea/phosphate lysis buffer", "reagent": ["Alizarin red S", "PBS"], "time": ["5 min"]}
{"action": "scrape", "output": "SDS-PAGE loading buffer", "reagent": ["100 \u03bcl urea/phosphate lysis buffer"]}
{"action": "dilute", "concentration": ["10 times"], "output": "", "reagent": ["SDS-PAGE loading buffer"]}
{"action": "quantify", "concentration": ["protein concentration"], "output": ""}
{"action": "evaluate", "output": "SDS-PAGE blot", "reagent": [""]}
{"action": "detect", "output": "", "reagent": ["IL-27", "biotinylated goat anti-mouse p28 Ab"]}