{"action": "centrifuge", "output": "", "speed": ["17,500 \u00d7 g"], "temperature": ["4 \u00b0C"], "time": ["1 h"]}
{"action": "remove", "output": "the pellets", "reagent": [""]}
{"action": "divide", "output": "the supernatants (soluble fractions)", "temperature": ["-80 \u00b0C"], "volume": ["10 \u00b5L"]}
{"action": "perform", "device": ["SDS-PAGE"], "output": "the BCA protein assay kit"}
{"action": "measure", "concentration": ["both positive cell lysates"], "output": "", "reagent": ["the BCA protein assay kit", "the supernatants (soluble fractions)"]}
{"action": "rinse", "output": "all future DNA library", "reagent": ["100 mM HCl"]}
{"action": "prepare", "output": "", "reagent": ["naive DNA library"], "volume": ["10 \u00b5L"]}
{"action": "use", "output": "", "reagent": ["all future DNA library", "this treatment"]}
{"action": "prepare", "concentration": ["100 \u00b5M"], "output": "", "reagent": ["temperature-treated DNA library (from step 6)"]}
{"action": "prepare", "concentration": ["10 \u00b5M DNA library"], "output": "DNA"}
{"action": "amplify", "output": "", "reagent": ["DNA"]}
{"action": "incubate", "output": "the DNA library", "temperature": ["room temperature"], "time": ["10 minutes"]}
{"action": "determine", "output": "the free DNA", "reagent": ["the DNA library"]}
{"action": "perform", "output": ""}
{"action": "determine", "output": "", "reagent": ["fluorescein", "the free DNA"]}