Freeze cells for 1 hour at -80°C, thaw at 37°C for 1 hour. #####%%%%% output "DLF_R004" are used in line4
{"action": "freeze", "output": "", "reagent": ["cells"], "time": ["1 hour"], "temperature": ["-80°C"]}
If not using DLF_R004, lyse cells with lysis buffer, .
{"action": "use", "output": "", "reagent": ["DLF_R004", "lysis buffer"]}
Prepare serological pipette by cutting at the 3 mL mark, sealing bottom with parafilm, .
{"action": "prepare", "output": "", "volume": ["the 3 mL mark"], "device": ["serological pipette"]}
Secure serological pipette to a vertical surface.
{"action": "secure", "output": "", "device": ["serological pipette"]}
Fill pipette with at least 2.5 mL cell lysate, measure distance from 2 mL to 1 mL mark.
{"action": "fill", "output": "", "volume": ["at least 2.5 mL"], "reagent": ["cell lysate"]}
Position cell phone camera to record pipette, drop a glass bead inside, repeat two more times.
{"action": "position", "output": "", "device": ["cell phone camera"]}
remove parafilm seal, ,
{"action": "remove", "output": "", "container": ["parafilm seal"]}
rinse pipette #####%%%%% output "next sample" are used in line18
{"action": "rinse", "output": "next sample", "device": ["pipette"]}
repeat with next sample to obtain triplicates #####%%%%% output "triplicates" are not used
{"action": "repeat", "output": "triplicates", "reagent": ["next sample"]}