{"action": "replace", "output": "Digest mESCs", "container": ["medium"], "volume": [""]}
{"action": "digest", "output": "mESCs", "reagent": ["0.05% trypsin"], "container": ["<<<96-well plates>>>"]}
{"action": "remove", "output": "", "container": ["<<<24-well plates>>>"]}
{"action": "confirm", "output": "positive colonies", "reagent": ["sgRNAs analysis (SPH primers)"]}
{"action": "replace", "output": "", "container": ["medium"], "volume": [""]}
{"action": "sort", "output": "single cells", "device": ["FACS"], "container": ["96-well plates"]}
{"action": "confirm", "output": "", "reagent": [""]}
{"action": "remove", "output": "", "container": ["24-well plates"]}
{"action": "confirm", "output": "positive colonies", "reagent": ["positive colonies"]}
{"action": "take", "output": "fluorescence images", "device": ["<<<confocal microscope>>>"], "container": ["colonies"]}