{"action": "collect", "container": ["culture medium"], "output": "", "volume": ["200 ml"]}
{"action": "centrifuge", "output": "crude exosome", "reagent": ["intact cells"], "speed": ["1,000 g"], "temperature": ["4\u00b0C"], "time": ["10 min"]}
{"action": "prepare", "concentration": ["10-90% sucrose solutions"], "output": "", "reagent": ["crude exosome"]}
{"action": "collect", "output": "", "volume": ["2 ml fractions"]}
{"action": "add", "mass": [""], "output": "", "volume": ["9 ml PBS"]}
{"action": "centrifuge", "output": "", "speed": ["100,000 g"], "time": ["1 hr,"], "volume": ["50 \u03bcl"]}
{"action": "include", "output": "exosomes", "reagent": [""]}