Harvest the infected spikelets from the plant by cutting them off using a pair of scissors.
{"action": "harvest", "output": "", "device": ["scissors"]}
Figure 1 shows the appearance of the infected spikelets.

Spikelets should be stored in a paper bag for ~10 days at 28 °C to dry out.
{"action": "store", "output": "", "container": ["paper bag"], "temperature": ["28 °C"]}
They can then be stored at room temperature until use.imgsrc:https://en-cdn.bio-protocol.org/attached/image/20170711/20170711204952_1040.jpgFigure 1.
{"action": "use", "output": "", "reagent": [""]}
The scale bar represents approximately 1 cm.
{"action": "provide", "output": "", "reagent": [""]}
The image background of the spikelets was digitally removed. #####%%%%% output "the contaminating spores that have germinated" are used in line20
{"action": "remove", "output": "the contaminating spores that have germinated", "container": ["the spikelets"]}
will not be damaged by the grinding

This will leave them more susceptible to the sterilisation treatment .

reduce overall contamination levels

This will kill most, if not all, of the contaminating spores that have germinated . #####%%%%% output "the spore mixture" are used in line24
{"action": "eliminate", "output": "the spore mixture", "reagent": ["the contaminating spores that have germinated"], "time": [""]}
not the U. bromivora spores which can take up to 17 h to germinate

Centrifuge the spore mixture at 1,200 x g for 5 min. #####%%%%% output "the spores" are used in line26
{"action": "centrifuge", "output": "the spores", "reagent": ["the spore mixture"], "force": ["1,200 x g"], "time": ["5 min"], "concentration": [""]}
Carefully pour out the liquid , on the third time, instead of re-suspending the spores in ddH2O, proceed to step 9.Re-suspend the spores in 300 µl ddH2O with antibiotics (ampicillin, ). #####%%%%% output "9.Re" are not used
{"action": "proceed", "output": "9.Re", "reagent": ["the spores"]}
These will kill any non-fungal cells that might have survived the CuSO4 treatment. #####%%%%% output "Tet, ChlA" are used in line30
{"action": "eliminate", "output": "Tet, ChlA", "reagent": ["CuSO4 treatment"], "time": [""]}
Plate 100 µl of each dilution on a PDAmp, Tet, ChlA plate (see Recipes), spread using glass beads . #####%%%%% output "Recipes" are not used
{"action": "spread", "output": "Recipes", "reagent": ["Tet, ChlA"]}