Resolve samples on 2% agarose gels using standard electrophoresis methods and reagents. Large wells are required to accommodate each 100 µL sample in a single well. 2. Using a UV transilluminator set to low intensity, excise the target band (~130 bp) from each lane, being careful to limit the UV exposure of each sample to no more than 30 seconds. 3. Extract preparations from excised gel slices either: A. using a commercial gel-extraction kit of your choice according to the manufacturer’s instructions; or B. by eluting directly from the gel slice into water (40 µL NFW in a 1.5 mL microcentrifuge tube). Gel slices are held overnight at 4°C, and the eluent collected the following morning. 4. Collect a small aliquot (~2-10 µL) from each preparation and combine in a multiplex pool for sequencing. Remaining preparations can be held for 6 months at -20°C and sampled again for sequencing if additional coverage is required for any samples.