{"action": "spin", "output": "", "speed": ["500-1,000 x g"], "time": ["10 min"]}
{"action": "add", "output": "", "reagent": ["supernatant"], "volume": ["4 ml XBP buffer"]}
{"action": "add", "container": ["exoEasy maxi spin column"], "output": "flow-through", "reagent": ["sample/XBP mix"]}
{"action": "centrifuge", "device": ["spin column"], "output": "Qiazol", "speed": ["5,000 x g"], "time": ["5 min"], "volume": ["10 ml XWP"]}
{"action": "add", "output": "", "reagent": ["Qiazol"]}
{"action": "add", "container": ["PLG tube"], "output": "", "reagent": ["flow-through"]}
{"action": "add", "output": "", "volume": ["90 \u00b5L chloroform"]}
{"action": "shake", "output": "", "temperature": ["RT"], "time": ["15 s"]}
{"action": "transfer", "container": ["new tube"], "output": "upper aqueous phase"}
{"action": "add", "output": "", "reagent": ["upper aqueous phase"], "volume": ["2 volumes"]}
{"action": "centrifuge", "container": ["MinElute spin column"], "output": "", "speed": ["1,000"], "time": ["15 s"]}
{"action": "centrifuge", "output": "", "speed": [">=8,000"], "time": ["15 s"], "volume": ["700 \u00b5L"]}
{"action": "centrifuge", "output": "RNase-free", "speed": [">=8,000"], "time": ["15 s"], "volume": ["500 \u00b5L Buffer RPE"]}
{"action": "add", "output": "RNA", "reagent": ["RNase-free"]}
{"action": "centrifuge", "output": "", "reagent": ["RNA"], "speed": [""], "time": [""]}