<AddSolid vessel="reactor" reagent="Z-Hyp-OH" mass="796 mg" />
<AddSolid vessel="reactor" reagent="PPh3" mass="942 mg" />
<ResetHandling solvent="[diethyl ether]" />
<ResetHandling solvent="THF" />
<EvacuateAndRefill vessel="reactor" />
<Dissolve vessel="reactor" solvent="THF" volume="9.61 mL" time="[60 s]" />
<Add vessel="DIAD_vial" reagent="THF" volume="5.14 mL" />
<HeatChillToTemp vessel="reactor" temp="[0 °C]" stir="True" />
<Transfer from_vessel="DIAD_vial" to_vessel="reactor" volume="8 mL" time="15 min" rinsing_volume="1.7 mL" rinsing_repeats="2" rinse_withdrawal_excess="0.0" />
<ResetHandling solvent="THF" />
<ResetHandling solvent="diethyl ether" />
<HeatChill vessel="reactor" temp="[35 °C]" time="8 h" stir="[True]" />
<Evaporate vessel="reactor" temp="45 °C" time="1 h" pressure="[50 mbar]" mode="auto" />
<Dissolve vessel="reactor" solvent="diethyl ether" volume="20 mL" time="[5 min]" />
<Transfer from_vessel="reactor" to_vessel="rotavap" volume="all" rinsing_volume="20 mL" rinsing_repeats="[3]" rinse_withdrawal_excess="0.0" />
<ResetHandling solvent="diethyl ether" />
<Evaporate vessel="rotavap" time="30 min" />
<Dry vessel="rotavap" time="30 min" />
<ResetHandling solvent="methanol" />
<ResetHandling solvent="ethyl acetate" repeats="[3]" volume="3 mL" />
<ResetHandling solvent="toluene" repeats="3" volume="[3 mL]" />
<Add reagent="toluene" vessel="[rotavap]" volume="10.3 mL" />
<ResetHandling solvent="[ethyl acetate]" repeats="3" volume="3 mL" />
<Dissolve solvent="ethyl acetate" vessel="rotavap" time="[5 min]" volume="2.57 mL" temp="[30 °C]" />
<PreparativeChromatography chromatography_system="C815" crude_material_vessel="rotavap" crude_material_volume="20 mL" washing_solvent="toluene" washing_volume="6 mL" additional_washing_solvent_1="ethyl acetate" additional_washing_volume_1="1 mL" washing_repeats="2" cleaning_solvent_1="methanol" clean_product_vessel="True" cleaning_repeats="2" product_vessel="rotavap" column="column_1" baseline_correction="False" equilibration_gradient_coordinates="0, 90, 10, 0, 0, 5, 90, 10, 0, 0" gradient_coordinates="0, 90, 10, 0, 0, 6, 60, 40, 0, 0, 8, 60, 40, 0, 0, 12,40, 60, 0, 0, 17,40, 60, 0, 0" use_detectors="['ELSD']" detector_thresholds="[45]" start_collection_after_cv="7" stop_collection_after_cv="13" />
<Evaporate vessel="[rotavap]" mode="auto" temp="[45 °C]" time="30 min" />
<Evaporate vessel="rotavap" mode="[auto]" temp="[45 °C]" time="30 min" />
<Dry vessel="[rotavap]" temp="45 °C" time="[30 min]" />
<ResetHandling solvent="ethyl acetate" repeats="3" volume="3 mL" />
<ResetHandling solvent="[methanol]" repeats="3" volume="3 mL" />