{"action": "dilute", "output": "", "reagent": ["PBS", "blood"]}
{"action": "collect", "device": ["Pasteur pipette"], "output": "interphase", "reagent": ["WBC"]}
{"action": "pellet", "output": "", "reagent": ["cells"], "speed": ["300 x g"], "temperature": ["RT"], "time": ["10 min"]}
{"action": "add", "output": "", "reagent": ["PBS"], "volume": ["50 ml"]}
{"action": "take", "output": "FACs analysis", "volume": [""]}
{"action": "centrifuge", "output": "", "speed": ["300 x g"], "temperature": ["RT"], "time": ["10 min"]}
{"action": "suspend", "output": "", "reagent": ["pellet"], "volume": ["300 \u00b5l"]}
{"action": "incubate", "output": "", "temperature": ["4 \u00b0C"], "time": ["10 min (RT)"]}
{"action": "charge", "device": ["LS column"], "output": "", "reagent": ["MACS buffer"], "volume": ["3 ml"]}
{"action": "re-suspend", "output": "", "reagent": ["cells"], "volume": ["500 \u00b5l"]}
{"action": "count", "output": "", "reagent": ["cells"]}
{"action": "pellet", "output": "", "reagent": ["cells"], "speed": ["400 x g"], "time": ["5 min"]}
{"action": "incubate", "output": "directly conjugated", "temperature": ["RT"], "time": ["10 min"]}
{"action": "add", "output": "", "reagent": ["directly conjugated antibody"]}
{"action": "suspend", "output": "", "volume": ["500 \u00b5l"]}
{"action": "proceed", "device": ["FACS analysis"], "output": ""}