{"action": "dissect", "output": "", "reagent": ["tissues"]}
{"action": "patch", "output": "", "reagent": ["4% PFA"], "temperature": ["room temperature"], "time": ["4 h"]}
{"action": "dehydrate", "output": "", "reagent": ["30% sucrose solution"]}
{"action": "prepare", "container": ["thin tissue sections"], "device": ["Cryostat microtome"], "output": ""}
{"action": "wash", "output": "samples", "reagent": ["0.3% Triton X-100 in PBS (PBST)"]}
{"action": "wash", "output": "HCR amplification buffer", "reagent": ["samples"]}
{"action": "incubate", "output": "secondary antibodies", "reagent": ["HCR amplification buffer"], "temperature": [""], "time": [""]}
{"action": "detect", "output": "samples", "reagent": ["secondary antibodies"]}
{"action": "wash", "output": "", "reagent": ["samples"]}
{"action": "add", "concentration": ["12.5 nM"], "container": ["amplification buffer"], "output": "GO", "reagent": [""]}
{"action": "add", "concentration": ["20 \u00b5g/mL"], "output": "samples", "reagent": ["GO"]}
{"action": "wash", "output": "", "reagent": ["samples"]}