If testing fungal isolate virulence, a mock treatment is also required.
{"action": "allow", "output": "", "reagent": ["fungal"]}
are allowed to germinate for 2 days whereas wheat is allowed to germinate for 3 days prior to inoculation
{"action": "allow", "output": ""}
Keep in dark by wrapping plates in aluminium foil. #####%%%%% output "each germinated seed" are used in line8
{"action": "keep", "output": "each germinated seed", "temperature": [""], "reagent": [""]}
Place mycelial plugs upside down on the main root of each germinated seed when about 3 cm long, at approximately 1 cm from the seed, with the mycelium in direct contact with the root (Figure 2).imgsrc:protocol.org/attached/image/20130404/20130404181439_2325.jpgFigure 2.
{"action": "place", "output": "", "reagent": ["mycelial plugs", "each germinated seed"]}