{"action": "amplify", "output": "", "reagent": ["the barcoded insert"]}
{"action": "go", "output": "the Qiagen\u00ae PCR Purification Kit", "reagent": [""]}
{"action": "pool", "output": "Lentiviral Plasmid", "reagent": ["the Qiagen\u00ae PCR Purification Kit"]}
{"action": "digest", "output": "digested products", "reagent": ["Lentiviral Plasmid", "Lentiviral Plasmid", "XmaI"]}
{"action": "run", "output": "Qiagen\u00ae MinElute Gel Extraction Kit (Qiagen, 28604)", "reagent": ["digested products"]}
{"action": "extract", "output": "H20", "reagent": ["Qiagen\u00ae MinElute Gel Extraction Kit (Qiagen, 28604)"]}
{"action": "elute", "output": "Lentiviral Plasmid", "reagent": ["H20"], "volume": ["25 l"]}
{"action": "ligate", "output": "", "reagent": ["Lentiviral Plasmid", "Ligation Buffer", "barcoded insert"]}
{"action": "read", "output": "the ligation product", "time": [""]}
{"action": "purify", "device": ["the Qiagen\u00ae PCR Purification Kit (Qiagen, 28106) 8"], "output": "", "reagent": ["the ligation product"]}
{"action": "transform", "output": "", "reagent": ["ElectroMAX DH10B cells", "Thermo Fisher, 18290015"]}
{"action": "provide", "output": "colonies", "reagent": [""]}
{"action": "peel", "container": ["plate"], "output": "", "reagent": ["", "LB-Amp", "bacterial colonies"]}
{"action": "determine", "concentration": ["plasmid concentration"], "output": "Sanger"}
{"action": "confirm", "output": "", "reagent": ["Sanger"]}