{"action": "incubate", "output": "", "reagent": ["EDTA", "antigen"], "temperature": ["<<<95\u00b0C>>>"], "time": ["50 min"]}
{"action": "rotate", "output": "H2O", "temperature": ["room temperature"], "time": ["20 min"]}
{"action": "wash", "output": "", "reagent": ["<<<H2O>>>"]}
{"action": "rotate", "output": "TBS", "reagent": ["3% H2O2"], "temperature": ["<<<room temperature>>>"], "time": ["5 min"]}
{"action": "wash", "output": "", "reagent": ["<<<TBS>>>"]}
{"action": "rotate", "output": "Ab mix", "temperature": ["room temperature"], "time": ["20 min"]}
{"action": "incubate", "output": "TBS", "reagent": ["anti-\u03b3H2AX", "Ab mix"], "temperature": ["4\u00b0C"]}
{"action": "wash", "output": "secondary antibody", "reagent": ["TBS"]}
{"action": "incubate", "output": "TBS", "reagent": ["secondary antibody"], "temperature": ["room temperature"], "time": ["30 min"]}
{"action": "wash", "output": "peroxidase substrate solution", "reagent": ["TBS"]}
{"action": "incubate", "output": "positive (irradiated skin)", "reagent": ["peroxidase substrate solution"], "temperature": ["room temperature"], "time": ["5 min"]}
{"action": "include", "output": "", "reagent": ["positive (irradiated skin)"]}
{"action": "repeat", "output": "the staining steps", "reagent": [""]}