{"action": "cut", "output": "", "device": ["<<<a cryostat>>>"]}
{"action": "display", "output": "", "time": [""], "string": [""]}
{"action": "rotate", "output": "", "temperature": ["<<<-20\u00baC>>>"]}
{"action": "remove", "output": "the blocking solution", "reagent": [""]}
{"action": "prepare", "output": "", "reagent": ["<<<the blocking solution>>>"]}
{"action": "incubate", "output": "1:5,000 rabbit anti-GFP (abcam ab290) -NeuN (Millipore ABN90P)", "reagent": ["the blocking solution"], "time": ["<<<1 hour>>>"], "temperature": [""]}
{"action": "make", "output": "", "reagent": ["1:5,000 rabbit anti-GFP (abcam ab290) -NeuN (Millipore ABN90P)"]}
{"action": "perform", "output": "anti-mouse AlexaFluor647", "frequency": ["three times"], "reagent": [""]}
{"action": "include", "output": "synaptic co-localization", "reagent": ["anti-mouse AlexaFluor647"]}
{"action": "add", "output": "", "reagent": ["hoechest (1:1,000)"]}