{"action": "set", "output": "primers", "reagent": ["template DNA (82.5 ng)", "10 mM Tris-HCl"], "volume": ["<<<20 \u00b5l>>>"]}
{"action": "run", "output": "", "reagent": [""], "temperature": ["94\u00b0C", "94\u00b0C", "60\u00b0C", "72\u00b0C"], "time": ["2 min", "1 min", "1 min", "1.75 min"]}
{"action": "detect", "output": "germline alleles", "reagent": ["DH-JH recombination", "primers", "DHL5\u2019"]}
{"action": "detect", "output": "", "reagent": ["germline alleles", "primers"]}