{"action": "transfect", "output": "stable clones", "reagent": ["ISX fusion GFP expression"]}
{"action": "generate", "output": "", "reagent": ["clones"]}
{"action": "prepare", "concentration": ["0.6% agar"], "output": ""}
{"action": "keep", "output": "", "temperature": ["65\u00b0C"]}
{"action": "repeat", "output": "the preparation steps", "time": [""]}
{"action": "dilute", "concentration": ["1 x 10^4 cells/ml", "20% FBS"], "output": ""}
{"action": "mix", "concentration": ["0.6% agar"], "output": "", "reagent": ["stable clones"]}
{"action": "add", "concentration": ["10% FBS MEM culture medium"], "output": "the medium addition", "volume": ["2 ml"]}
{"action": "repeat", "output": "", "reagent": ["the medium addition"]}
{"action": "repeat", "output": "the staining process", "time": [""]}